Although manual patch clamping and automated patch clamping using the QPatch systems generate data with identical high quality, the preparation procedures of the target cells remain very different. While the experimentor in manual patch clamp experiments often has a reporting system for selection of high expression cells, the automated system generates whole-cells by random selection of cells.
The implications are very significant: for automated patch clamp careful cell culture routines must be implemented to yield a uniform cell line with a near perfect and stable expression profile over the entire cell population for as many passages as possible. With our unique experience with automated patch clamping and cell culture optimizations, we offer two options to get the most of you cell lines: Custom cell line optimizations, or sale of QPatch optimized cell lines.
Custom cell line optimization
Sophion Bioscience offers the service of optimizing your cell line of interest. Ship your cell line to Sophion and we will optimize cell culture procedures and assay parameters for you. Everything is of course conducted under strict confidentiality. A cell line optimization takes as little time as one to two months. In return you get:
. 10 vials of optimized frozen cells
. Optimized SOP for cell culturing
. QPatch Assay protocols
Validation report containing:
. Ringer’s solution recommendations
. Validation of electrophysiological ion channel properties
. Success rate data
Following your cell line optimzation, your designated Application Scientist will be available for all necessary follow-up questions and support via email or telephone, free of charge.
In addition to the full custom cell line optimization, Sophion Bioscience also offers the opportunity to rent-a-resource for training and tips on cell line and assay optimizations.
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