QPatch IC50 values for 32 unknown compounds provided by Aventis. In the original test, several compounds gave IC50 that were 5-15 times higher than those estimated by conventional patch-clamp (determined by Aventis). Because the compounds had been kept in plastic MTPs prior to the test, it was suggested that non-specific binding ('stickiness') could be the reason for the discrepancy. Consequently 11 of the compounds with markedly raised IC50 values were retested after being kept in glass-coated MTPs. These data are shown in the diagram.
As a mean the QPatch IC50 values are a factor 1.15 higher than the manually determined values. The diagram also includes IC50 values for two reference compounds (rBeKm-1 and verapamil).
Contact Frank Henrichsen, fhe@sophion.dk, to get the entire report.
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