KCNQ2/3
Voltage dependent: KQT related
(Alternative nomenclature: Kv7.2/Kv7.3)
KCNQ2/3 is a slow delayed rectifier voltage-gated 6-TM 1-P type potassium channel. Heterotetrameric (KCNQ2 and KCNQ3 subunits).
Localization:
KCNQ2: brain (somatodendritic pyramidal and polymorphic neurons; cortex and hippocampus), sympathetic ganglia, testis.
KCNQ3: brain (somatodendritic pyramidal and polymorphic neurons; cortex and hippocampus), sympathetic ganglia, spleen, cochlea
Information links:
http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602235 (KCNQ2)
http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602232 (KCNQ3)
QPatch recording of KCNQ2/3 currents:
KCNQ2/3 co-expressed in HEK293 cells.
Currents elicited in response to depolarizations from -90 mV to -30 mV.
Pharmacological profiling:
Inhibitors: Linopirdine
Activators: Retigabine, BMS-204352
(The lists are not intended to be complete)
QPatch concentration-response analysis:
(Courtesy of Dr. Mads P.G. Korsgaard, NeuroSearch)
Inhibition of KCNQ2/3 whole-cell currents by the irreversible inhibitor DMP543. Blue trace: control. Red trace: 3 μM DMP543. Turquoise and pink traces: subsequent to wash out.
Effect of the activator retigabine on KCNQ2/3 whole-cell currents. Following control (blue trace) two concentrations (1 and 10 μM) of retigabine were added (red and purple traces, respectively).
I-t plot of the KCNQ2/3 whole-cell current data shown above.
Pathophysiology:
Benign neonatal epilepsy (EBN1)
QPatch written material:
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