Sophion Bioscience

Na_v1.2
Voltage dependent sodium channel

(Alternative nomenclature: SCN1A, NAC2)

Na_v1.2 is a 24-TM (four 6-TM repeats) type voltage-gated sodium channel.

Localization:
Most abundant form in brain. Peripheral nerve (initial segment; nodes of Ranvier)

Information links:
http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=182390
http://www.expasy.org/cgi-bin/niceprot.pl?Q99250

QPatch recordings of Na_v1.2 currents:

Whole-cell Na_v1.2 current recordings. Voltage protocol: 20 ms depolarization to 0 mV from a holding potential of –90 mV. Expression system HEK-293.

Current-voltage relationship for peak whole-cell Na_v1.2 sodium currents.

Pharmacological profiling:
Inhibitors: tetrodotoxin, saxitoxin
Activators: N/A
(The lists are not intended to be complete)

QPatch concentration-response analysis:

Whole-cell Na_v1.2 currents in response to increasing concentrations (0.5-500 nM) of tetrodotoxin
(TTX).

Concentration-response relationship for TTX based on the whole-cell Na_v1.2 currents presented above. IC₅₀ = 9.4 nM.

Pathophysiology:
Seizure disorders (generalized eplieysy with febrile seizures plus)

Recent review articles:
Burgess DL. Neonatal epilepsy syndromes and GEFS+: mechanistic considerations. Epilepsia; 46 Suppl 10:51-8, 2005.

QPatch written material:

Application reports:
Biophysical characterization of fast voltage-gated sodium channels by QPatch 16 (339 kB)

Posters:
Pharmacological Characterization of Voltage- and Ligand-gated Ion Channels by QPatch, Japanese Pharmacological Meeting 2006 (424 kB)

Biophysical and pharmacological profiling of multiple NA_v subtypes on QPatch (1.4 MB)

Articles:
Increased throughput in ion channel drug development and exploration by automation of electrophysiology. American Biotechnology Laboratory 24: issue 4, 20-21, 2006 (2.87 MB)

page top